CGSC Strain#: 8209
Strain Designation: BW18813/pBW30     
Source of Strain: B.L. Wanner
Sex: F-
Episome/Plasmid: pBW30
Plasmid Markers/Mutations: oriR6Kγ,
bla(ApR)
,
oriTRP4,
bla ,
oriRg Chromosomal Markers: Δ(codB-lacI)3,
ΔphoA532,
ΔuidA4::pir-116,
rpoS396(Am)
,
rph-1,
Δ(phnP-phnD)3330( phnC?),
[phnB]Strain Comments: - pBW30 is the same as pLD54 except for PstI site in bla. pLD54 host is pir+, recA1, lacIQ and CamR.
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- ΔuidA4::pir-116-- Allows plasmids with an R6Kγ origin to replicate to high copy number
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- [phnB]-- functional phn locus from E. coli B
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- Am = amber(UAG) mutation
- phnC? = may also delete phnC
- ApR = ampicillin resistant
References: