CGSC Strain#: 5952
Strain Designation: SB3931     
Source of Strain: P.E. Hartman
Other Designations: UTH860
Sex: F-
Chromosomal Markers: araC14,
glnX44(AS)
,
galK2(Oc)
,
λ-,
hisF860(Stable)
,
rpsL145(strR)
,
malT1(λ
R)
,
xylA5,
mtl-1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- Stable = stable, non-reverting
- strR = streptomycin resist.
References: - Goldschmidt, E.P., M.S. Cater, T.S. Matney, M.A. Butler, A. Greene 1970. Genetic analysis of the histidine operon in Escherichia coli K12. Genetics 66:219-229
- Carl, PL 1970. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol. Gen. Genet. 109(2):107-22.