CGSC Strain#: 6785
Strain Designation: MM28     
Source of Strain: M. Meselson
Other Designations: M28
Other Designations: N99(NIH)
Sex: F-
Chromosomal Markers: galK2(Oc)
,
λ-,
IN(rrnD-rrnE)1,
rpsL200(strR)
,
rph-1Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- galK2(Oc) was formerly called gal2
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Gottesman, M.E., M. Yarmolinsky 1968. Integration-negative mutants of bacteriophage lambda. J.Mol.Biol. 31:487-505
- Pratt, D., W.S. Erdahl 1968. Genetic control of bacteriophage M13 DNA synthesis. J.Mol.Biol. 37:181-200