CGSC Strain#: 7729
Strain Designation: ALS225      Source of Strain: E. Altman
Sex: F' Episome/Plasmid: F128-xxx
   Plasmid Markers/Mutations:
Chromosomal Markers: Δ(araA-leu)7697, [araD139]_B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ^-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2
Strain Comments:

• Δ(araA-leu)7697-- Shown by Durfee et al. to be a deletion of the first 295 codons of hepA to the first 82 codons of fruR.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• galK16-- An IS2 insertion resulting in the truncation of the protein at codon 56
• galE15(GalS)-- is Brenner's gal^-_U38, isolated by UV mutagenesis from his strain CA1.
• galE15(GalS)-- G to A transition resulting in an S122F amino acid substitution
• e14--- : Loss of the e14 element makes the strain McrA^-.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(araA-leu)7697 was formerly called Δ(hepA-fruR)7697
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• GalS = UDPGal accum.toxic if GalKRP+
• strR = streptomycin resist.

References:

• Warren, J., J. Walker, J.R. Roth, E. Altman 2000. Construction and characterization of a highly regulable expression vector pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33. Plasmid 44:138-141