CGSC Strain#: 5215
Strain Designation: ND40      Source of Strain: E. Ohtsubo
Sex: F' Episome/Plasmid: F100-13
   Plasmid Markers/Mutations: glnV0(AS)
Chromosomal Markers: ΔlacZ39, λ^-, trpA49(Am), recA11, relA1, rpsL150(strR), spoT1
Strain Comments:

• Note the suprressor mutation on F100-13. This may be supE44. DE lacZ39 is a deletion of ~ 1/3 of lacZ. It has also been called lac4680.
• ΔlacZ39-- is a deletion of ca. 1/3 of the lacZ gene.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• AS = amber(UAG) suppressor

References:

• Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.