CGSC Strain#: 8218
Strain Designation: BW19335/pWM93     
Source of Strain: B.L. Wanner
Sex: F-
Episome/Plasmid: pWM93
Plasmid Markers/Mutations: oriR6Kγ,
bla(ApR)
,
oriTRP4,
lacZα,
[sacB]subtilis,
orif1,- Plasmid Comment: Same as pWM91, except ori_f1(-)-lacZ from pBluescript II (SK-)
Chromosomal Markers: Δ(codB-lacI)3,
ΔphoA532,
ΔuidA3::pir+,
Δ(phnP-phnD)3330( phnC?)Strain Comments: - Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- ΔuidA3::pir+-- Allows the replication of plasmids with an R6Kγ origin of replication
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- ΔuidA3::pir+ was formerly called uidA(Δ MluI)::pir+
- phnC? = may also delete phnC
- ApR = ampicillin resistant
References: - Alting-Mees, M.A., J.A. Sorge, J.M. Short 1992. pBluescriptII: Multifunctional cloning and mapping vectors. Methods Enzymol. 216:483-495
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.