CGSC Strain#: 5221
Strain Designation: M5154      Source of Strain: E.R. Signer
Other Designations: KRO
Sex: F- Chromosomal Markers: ΔlacZ39, λ^-, trpA49(Am), recA11, relA1, rpsL150(strR), spoT1
Strain Comments:

• ΔlacZ39-- is a deletion of ca. 1/3 of the lacZ gene.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Zissler, J., E. Signer, F. Schaefer 0. The role of recombination in growth of bacteriophage lambda. I. The gamma gene, p. 455-468. In A.D. Hershey (ed.), The bacteriophage lambda. In: The Bacteriophage Lambda
• Mertz, JE, ER Signer, F Schaefer 1975. Amber red- mutants of phage lambda. Virology 63(2):591-5.