CGSC Strain#: 7970
Strain Designation: DK552      Source of Strain: D. Kennell
Sex: F- Episome/Plasmid: pJM(pUC18::rna)
   Plasmid Markers/Mutations: bla(ApR), rna(Ω6.5kb), rna
   Plasmid Comment: 6.5 kb R1-BamH1 fragment from Kohara No. 166 clone, in pUC18
Chromosomal Markers: proC29, rna-30::kan, λ^-, relA1, spoT1, metB1, mcrB1
Strain Comments:

• Overproduces RNase I. Preferable to DK523 for large-batch cultures because 2 resistance markers gives more protection from contamination.
• rna-30::kan-- is a kan insertion 322 bp from start of rna coding region.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• proC29 was formerly called pro3-52
• proC29 was formerly called proC52
• = kanamycin resistant
• ApR = ampicillin resistant

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