CGSC Strain#: 7065
Strain Designation: MRi80     
Source of Strain: J. Lopilato
Sex: F-
Chromosomal Markers: [araD139]B/r,
pcnB80,
Δ(argF-lac)169,
λ-,
flhD5301,
Δ(fruK-yeiR)725(fruA25),
relA1,
rpsL150(strR),
Δrbs-7,
deoC1Strain Comments: - [araD139]B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
- Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- flhD5301-- flagellar defect shown in Komeda and Iino
- Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
- fruA25 = former designation
- strR = streptomycin resist.
References: - Lopilato, J., S. Bortner, J.R. Beckwith 1986. Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol.Gen.Genet. 205:285-290