CGSC Strain#: 7972
Strain Designation: NM81      Source of Strain: S. Schuldiner
Sex: F- Chromosomal Markers: thr-1, ΔnhaA1::kan, Δ(lacY-lacZ)9998, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10
Strain Comments:

• To avoid sensitivity to Na⁺ at pH above 7.5 in this nhaA^- nhaB⁺ strain, LBK medium, which substitutes KCl (6.5 g/l) for NaCl in LB medium, may be used.
• melB-(ts,LiD)-- allows growth on melibiose at 30 C, but not at 37 C; after several days at 37 C, spontaneous revertants will arise.
• = kanamycin resistant
• = tetracycline resistant
• LiD = lithium dependent
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Padan, E., N. Maisler, D. Taglicht, R. Karpel, S. Schuldiner 1989. Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter systems(s). J.Biol.Chem. 264:20297-20302
• Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734