CGSC Strain#: 4963
Strain Designation: W3110 ColE3, ColI, StrR     
Source of Strain: D. Helinski
Sex: F-
Episome/Plasmid: ColE3
Plasmid Markers/Mutations: Episome/Plasmid: ColI
Plasmid Markers/Mutations: Plasmid Comment: ColI from CA38 (Fredericq)
Chromosomal Markers: λ-,
IN(rrnD-rrnE)1,
rpsL149(strR)
,
rph-1Strain Comments: - This strain produces the I-type colicin in extremely low amount. ColE3 and ColI came from CA38 (P.Fredericq)
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- strR = streptomycin resist.
References: - Herschman, H.R., D. Helinski 1967. Comparative study of the events associated with colicin induction. J.Bacteriol. 94:691-699