CGSC Strain#: 4977
Strain Designation: 3.300 U281      Source of Strain: Paris Strain
Other Designations: CA86
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ281(Am), lacI22, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• lacZ281(Am) was formerly called lacZ_U281
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References: