CGSC Strain#: 4979
Strain Designation: CA161      Source of Strain: S. Brenner
Other Designations: CA161
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ53(Am), glnX44(AS), λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Signer, E., J.R. Beckwith, S. Brenner 1965. Mapping of suppressor loci in Escherichia coli. J.Mol.Biol. 14:153-166
• Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637