CGSC Strain#: 4813
Strain Designation: ER     
Source of Strain: E. Reich
Other Designations: ATCC#25287
Sex: F+
Chromosomal Markers: asnB32,
λ-,
relA1,
spoT1,
asnA31,
thiE1Strain Comments: - This strain requires 100ug/ml L-asparagine.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- asnA31-- Reported to be an amber mutation
- thiE1 was formerly called thi-1
References: - Cedar, H, JH Schwartz 1969. The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J. Biol. Chem. 244(15):4112-21.
- Felton, J, S Michaelis, A Wright 1980. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J. Bacteriol. 142(1):221-8.