CGSC Strain#: 4880
Strain Designation: DF71      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacI22, λ^-, e14-, eda-1, relA1, spoT1, thiE1
Strain Comments:

• When using eda as a selective marker, select growth on glucuronic acid because growth on gluconic acid selects for edd mutants which relieve the inhibition caused by eda.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• thiE1 was formerly called thi-1

References:

• Fradkin, J.E., D. Fraenkel 1971. 2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli. J.Bacteriol. 108:1277-1283