CGSC Strain#: 4875
Strain Designation: DF1070     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ompF627(T2R)
,
edd-1,
gnd-1,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Peyru, G, DG Fraenkel 1968. Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli. J. Bacteriol. 95(4):1272-8.
- Fraenkel, DG 1968. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J. Bacteriol. 95(4):1267-71.