CGSC Strain#: 4827
Strain Designation: G240     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoA7(UGA)
,
phoA10,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA7 is an opal mutation (UGA). phoA10 does not affect the activity of the enzyme.
- phoA10-- does not affect the activity of the enzyme.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- = missense
- Const = constitutive
- T2R = T2 Resistant
- UGA = opal(UGA) mutation
References: - Chan, T.S., A. Garen 1970. Amino acid substitutions resulting from suppression of nonsense mutations. V. Tyrptophan insertion by the Su9+ gene, a suppressor of the UGA nonsense triplet. J.Mol.Biol. 49:231-234