CGSC Strain#: 4878
Strain Designation: BJ502      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, tktA2, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• The tkt marker reverts. It must be checked at use.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Josephson, B.L., D.G. Fraenkel 1969. Transketolase mutants of Escherichia coli. J.Bacteriol. 100:1289-1295