CGSC Strain#: 3446
Strain Designation: AB3446     
Source of Strain: E.A. Adelberg
Sex: F'
Episome/Plasmid: F8-1
Plasmid Markers/Mutations: ts F Plasmid Comment: F8-1 is a derivative of F8 with a temperature sensitive mutation in the F genetic material.
Chromosomal Markers: λ-,
IN(rrnD-rrnE)1,
thi-2Strain Comments: - 1.At 30 degrees C this strain is relatively stable for gal+, but there is some episome loss. Consequently, it is necessary to reisolate FTS clones often. At 42 degrees C the
- 2.temperature-sensitive F8-1 is lost almost completely. When colonies are incubated for more than 48 hours at 42 degrees C, small red papillae appear on the surface. On subculture these appear to be
- 3.temperature-stable F' strains.
- Ref. below (Ohtsubo and Hsu) is to F8. Lyophil opened 8/15/02
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
References: - Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.