CGSC Strain#: 4017
Strain Designation: HfrH 165/70      Source of Strain: F. Bonhoeffer
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, relA1, thyA11, spoT1, thiE1, dnaB70(ts)
Strain Comments:

• Derivation given p.9.2. Also called X1006, p.9.2.
• thyA11-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• ts = Temperature Sensitive

References:

• Bonhoeffer, F 1966. DNA transfer and DNA synthesis during bacterial conjugation. Z. Vererbungsl. 98(2):141-9.