CGSC Strain#: 4213
Strain Designation: JC182     
Source of Strain: A.J. Clark
Other Designations: AB862
Sex: Hfr
Point of Origin#: 1, 12
Map Position: 96.71
Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ-,
e14-,
purF1,
relA1,
thiE1Strain Comments: - This is Clark's "double male" strain, formerly called "D2". The position of PO 1 is shown above; that of PO 12 is between metC and rpoD.
- e14--- : Loss of the e14 element makes the strain McrA-.
- purF1-- is a very stable mutation and may be an intragenic deletion.
- thiE1 was formerly called thi-1
References: - Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120
- Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
- Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15