KMBL1789      Source of Strain:
F- Chromosomal Markers: λ-
, deoB301Strain Comments:
- thyA305 mistakenly designated thyA301 in the paper below. bioA2 designated bio-87 in paper below.
- thyA305-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- = good miniprep dsDNA quality
- Glickman, BW, CA van Sluis, HL Heijneker, A Rörsch 1973. A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. I. General characterization. Mol. Gen. Genet. 124(1):69-82.