CGSC Strain#: 5769
Strain Designation: MI262     
Source of Strain: M. Iaccarino
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: leuB6(Am)
,
ilvI614,
ilvH612,
λ-,
relA1,
spoT1,
ilvB619,
ilvG603(Act)
,
ilvG605(Am)
,
thiE1Strain Comments: - This strain is Ilv-. It has a partial requirement for diaminopimelate and lyses at temperatures above 38 degrees C.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- ilvG603(Act)-- is an insertion mutation which activates the ilvG gene.
- ilvG605(Am)-- is an amber mutation.
- thiE1 was formerly called thi-1
- Act = activates the Gene
- Am = amber(UAG) mutation
References: - Guardiola, J, M De Felice, M Iaccarino 1974. Mutant of Escherichia coli K-12 missing acetolactate synthase activity. J. Bacteriol. 120(1):536-8.
- De Felice, M., J. Guardiola, W. Schreil, M. Levinthal, M. Iaccarino 1977. Metabolic interlock between the acetolactate synthase isoenzymes and lysine biosynthesis in Escherichia coli K-12. Mol.Gen.Genet. 156:9-16
- De Felice, M, C Squires, M Levinthal, J Guardiola, A Lamberti, M Iaccarino 1977. Growth inhibition of Escherichia coli K-12 by L-valine: a consequence of a regulatory pattern. Mol. Gen. Genet. 156(1):1-7.
- Lawther, R.P., D.H. Calhoun, J.E. Gray, C.W. Adams, C.A. Hauser, G.W. Hatfield 1982. DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J.Bacteriol. 149:294-298