CGSC Strain#: 6592
Strain Designation: pLC16-37/WL8     
Source of Strain: J. Walsh
Sex: F-
Episome/Plasmid: pLC16-37
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
melA Plasmid Comment: Use CGSC No. 6592. Hybridizes with Kohara clones 645-646.
Chromosomal Markers: recA56,
srlD300::Tn10,
relA1,
spoT1,
metB1,
melA7Strain Comments: - The plasmid carries the gene melA.
- recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- srlD300::Tn10 was formerly called srlC300::Tn10
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: