CGSC Strain#: 4210
Strain Designation: EM2001     
Source of Strain: E. McFall
Other Designations: F32
Sex: F'
Episome/Plasmid: F32
Plasmid Markers/Mutations: Chromosomal Markers: lacY1 or lacZ4,
glnX44(AS)
,
λ-,
rfbC1,
purF1,
aroC4,
dsdA1,
rpsL8 or rplL9(L?)
or rpsL14,
malT1(λ
R)
,
xyl-7,
mtlA2,
ΔargH1,
thiE1,
tsx-23 or tsx-25Strain Comments: - This strain should be grown on medium containing B1, arg, and lacking try, phe, tyr, ade.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- purF1-- is a very stable mutation and may be an intragenic deletion.
- dsdA1-- is called dsdA7 by McFall (1967 Genetics 55:91).
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - McFall, E 1967. Dominance studies with stable merodiploids in the D-serine deaminase system of Escherichia coli K-12. J. Bacteriol. 94(6):1982-8.