CGSC Strain#: 5449
Strain Designation: KY2053     
Source of Strain: T. Yura
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
secA208,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
λ-,
trp-67,
his-100,
rpsL135(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1,
met-99Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- secA208-- : azi is now secA
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- secA208 was formerly called azi-8
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Wada, C., T. Yura 1974. Phenethyl alcohol resistance in Eslcherichia coli. III. A temperature-sensitive mutation (dnaP) affecting DNA replication. Genetics 77:199-220