CGSC Strain#: 7779
Strain Designation: K554     
Source of Strain: Russel & Model
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
proC471::Tn5,
phoR86,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoR86-- Originally from Beckwith strain SM135, may be the same as phoR82
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- = kanamycin resistant
- Const = constitutive
- T2R = T2 Resistant
References: