CGSC Strain#: 7809
Strain Designation: K1718     
Source of Strain: Russel & Model
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
phoA4(Am)
,
λ-,
ompF627(T2R)
,
serU132(AS)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
ilvY864::Tn10,
rrnB-2,
mcrB9999,
mcrB1,
creC510Strain Comments: - phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- serU132(AS) was formerly called supD32
- serU132(AS) was formerly called Su1+
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Const = constitutive
- T2R = T2 Resistant
References: