CGSC Strain#: 4868
Strain Designation: K8-5m      Source of Strain: H.L. Kornberg
Sex: F- Chromosomal Markers: lacY1 or lacZ4, tsx-6, glnX44(AS), galK2(Oc), rpsL159(strR), xyl-7 or xylA5, metF24, aceA3, iclR13
Strain Comments:

• Kornberg reports that the met marker is metB or metF. iclR13 was discovered in this strain by Vinopal & Fraenkel.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Vinopal, RT, DG Fraenkel 1974. Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt. J. Bacteriol. 118(3):1090-100.