CGSC Strain#: 8256
Strain Designation: NCM534     
Source of Strain: N. Majdalani
Sex: F-
Chromosomal Markers: Δ(araD-araB)714,
Δ(lacA-lacZ)880(::FRT),
lacIp-4000(lacIQ),
zah-2225::FRT,
λ-,
ΔaraEp-532::FRT,
φPcp18araE533,
rph-1Strain Comments: - Δ(lacA-lacZ)880(::FRT)-- is the DE(lacA-lacZ)879::kan deletion with the kan cassette removed
- zah-2225::FRT-- is the same as zah-2224::cat with the cat cassette removed
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- ::FRT = Flp Recombinase Target
References: