CGSC Strain#: 5846
Strain Designation: JM1103      Source of Strain: M.C. Jones-Mortimer
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, galK2(Oc), λ^-, ompF627(T2R), ptsG23, Δ(his-gnd)79, mgl-50, fruA10, fadL701(T2R), relA1, thyA111, galP64, pitA10, spoT1
Strain Comments:

• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• thyA111-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• galK2(Oc) was formerly called gal₂
• Oc = ochre(UAA) mutation
• T2R = T2 Resistant

References:

• Henderson, PJ, RA Giddens, MC Jones-Mortimer 1977. Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochem. J. 162(2):309-20.