CGSC Strain#: 5850
Strain Designation: RK4536      Source of Strain: R.J. Kadner
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, lacZ36, proC32, tsx-67, purE42, glnX44(AS), λ^-, trpE38, rfbC1, gyrA90(NalR), lysA23, rpsL109(strR), xylA5, mtl-1, metE70, ΔargH1, rpoB308, thiE1, metH156
Strain Comments:

• Ref. to metH156 below. This strain is sensitive to 40ug/ml nalidixic acid.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• proC32 was formerly called proC65
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• NalR = Nalidix.acidResist.
• strR = streptomycin resist.

References:

• Kadner, R.J., Liggins 1973. Transport of vitamin B12 in Escherichia coli: genetic studies. J.Bacteriol. 115:514-521