CGSC Strain#: 6121
Strain Designation: MI248      Source of Strain: M. Iaccarino
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: brnS-??-7, lacZ53(Am), e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References:

• Guardiola, J., M. DeFelice, T. Klopotowski, M. Iaccarino 1974. Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J.Bacteriol. 117:393-405