CGSC Strain#: 6135
Strain Designation: EWH331      Source of Strain: E.W. Hafner
Sex: Hfr    Point of Origin#: 132(ColV.)   Map Position: 69.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-1, araC14, ΔspeD98, Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, Δ(speB-speA)97, Δ(speC-glcB)63, rpsE2128(SpcR), rpsL25(strR), xylA5, mtl-1, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• SpcR = spectinomycin resistant
• strR = streptomycin resist.

References:

• Hafner, EW, CW Tabor, H Tabor 1979. Mutants of Escherichia coli that do not contain 1,4-diaminobutane (putrescine) or spermidine. J. Biol. Chem. 254(24):12419-26.