CGSC Strain#: 2070
Strain Designation: AB2070      Source of Strain: G. Eggertsson
Sex: F- Chromosomal Markers: ara-19, fhuA1, Δ(gpt-proA)62, lacY1 or lacZ4, tsx-33, glnX44(AS), galK2(Oc), λ^-, Rac-0, hisG4(Oc), rfbC1, rpsL8 or rplL9(L?), malT1(λ^R), mtl-1, ilvE12, metE46, thiE1
Strain Comments:

• This strain is stimulated by leucine under some conditions.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Eggertsson, G., E.A. Adelberg 1965. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics 52:319-340
• Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194
• Marsh, NJ, DE Duggan 1972. Ordering of mutant sites in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F 14 : a new procedure for fine-structure mapping. J. Bacteriol. 109(2):730-40.