CGSC Strain#: 3645
Strain Designation: CR34/399      Source of Strain: F. Bonhoeffer
Other Designations: AB3645
Other Designations: PB31
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, rfbC1, thyA6, dnaG399(ts), rpoD800(ts), rpsL67(strR), thiE1, deoC1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• rpoD800(ts)-- is a temperature-sensitive mutation.
• dnaG399(ts) was formerly called tsm-6
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Marinus, MG, EA Adelberg 1970. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. 104(3):1266-1272.
• Gross, J. 1972. Curr.Topics Microbiol.Immunol. 57:39-0
• Liebke, H., C.A. Gross, W. Walter, R.R. Burgess 1980. A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants. Mol.Gen.Genet. 177:277-282