CGSC Strain#: 3659
Strain Designation: 2000 X111F
TS62-lac
+     
Source of Strain: Cuzin & Jacob
Sex: F'
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
λ-,
relA1,
spoT1,
thiE1Strain Comments: - gpt-lac5 has been called DE proB-lacX111.
- Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
References: - Beckwith, J.R., E.R. Signer 1966. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi 80. J.Mol.Biol. 19:254-265
- Cuzin, F., F. Jacob 1967. Ann.Inst.Pasteur 112:397-0