CGSC Strain#: 3677
Strain Designation: 2000X111F_TS114-lac⁺      Source of Strain: Cuzin & Jacob
Sex: F' Episome/Plasmid: F42-114
   Plasmid Markers/Mutations: F_ts114, lac
   Plasmid Comment: Temperature sensitive mutation on the F.
Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), λ^-, relA1, spoT1, thiE1
Strain Comments:

• DE gpt-lac5 has been called DE (proB-lac)X111.
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor

References:

• Beckwith, J.R., E.R. Signer 1966. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi 80. J.Mol.Biol. 19:254-265
• Cuzin, F., F. Jacob 1967. Ann.Inst.Pasteur 112:397-0