CGSC Strain#: 4132
Strain Designation: AB4132     
Source of Strain: E.A. Adelberg
Sex: F-
Chromosomal Markers: araC14,
lacY1,
tsx-57,
glnX44(AS)
,
galK2(Oc)
,
alaS4(ts)
,
metC56,
xyl-7 or xylA5,
thiE1,
tfr-5Strain Comments: - alaS4 is a temperature-sensitive mutation.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- ts = Temperature Sensitive
References: - Theall, G, KB Low, D Soll 1977. Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity. Mol. Gen. Genet. 156(2):221-7.