CGSC Strain#: 4233
Strain Designation: Hfr R5      Source of Strain: P.R. Reeves
Other Designations: Reeves 5
Other Designations: CSH68
Sex: Hfr    Point of Origin#: 47   Map Position: 2.71   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: lacY1, glnX44(AS), gal-3, rfbC1, malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• The gal-3 may consist of two mutations and one suppressor.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167