CGSC Strain#: 4237
Strain Designation: Hfr Broda 7      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 43   Map Position: 31.58   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, relA1, spoT1, metB1, creC510, λ^R
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• λ^R-- Lambda resistant, locus unknown.
• creC510 was formerly called phoM510
• Const = constitutive

References:

• Broda, P. 1967. The formation of Hfr strains in Escherichia coli K12. Genet.Res. 9:35-47
• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Sparling, P.F., E. Blackman 1973. Mutation to erythromycin dependence in Escherichia coli K-12. J.Bacteriol. 116:74-83