CGSC Strain#: 6514
Strain Designation: CT28-3b     
Source of Strain: Schubach et al.
Other Designations: JW226
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
glnX44(AS)
,
galK2(Oc)
,
λ-,
thyA705,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
dnaC28(ts)
Strain Comments: - This strain should be grown at 25C.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- thyA705-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- dnaC28(ts)-- is a temperature-sensitive mutation.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Schubach, WH, JD Whitmer, CI Davern 1973. Genetic control of DNA initiation in Escherichia coli. J. Mol. Biol. 74(2):205-21.