CGSC Strain#: 6209
Strain Designation: KL363      Source of Strain: S. Morgan
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), galK2(Oc), recA1, metC56, rpsL69(strR), glnT4(ts), xyl-7 or xylA5, lldD1, thiE1, valS7(ts)
Strain Comments:

• The mutation gltnT4 renders this strain temperature-sensitive.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• valS7(ts)-- is a temperature-sensitive mutation. Growth stops at 35 degrees C.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Morgan, S., A. Korner, K.B. Low, D. Soll 1977. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. I. Isolation and characterization of a mutant with elevated levels of tRNA_1^Gln. J.Mol.Biol. 117:1013-1031