CGSC Strain#: 6374
Strain Designation: JK84     
Source of Strain: J. Parker
Sex: F-
Chromosomal Markers: lacY1 or lacZ4,
glnX44(AS)
,
λ-,
hisS210(ts)
,
glyA6,
relA1,
rpsL8 or rplL9(L?)
or rpsL14,
xyl-7,
mtlA2,
ΔargH1,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisS210(ts)-- The requirement for His is leaky at 30 degrees C and tight at 37 degrees. The marker reverts.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- AS = amber(UAG) suppressor
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Parker, J, SE Fishman 1979. Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli. J. Bacteriol. 138(1):264-7.