CGSC Strain#: 6419
Strain Designation: SK2210     
Source of Strain: S.R. Kushner
Sex: F-
Chromosomal Markers: Δ(lacY-lacZ)286,
tsx-3,
glnX44(AS)
,
λ-,
lacZ95=phi80dIIlacZ9,
hisG4(Oc)
,
rpsL281(strR)
,
xyl-7,
mtl-1,
rbsD296::Tn10,
ΔargH1Strain Comments: - Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
- rbsD296::Tn10-- cotransduces 68% with ilv, counterclockwise. Shown by PCR analysis to be within the rbsD gene at nucleotide 3,931,475 (84.744 min.)
- Δ(lacY-lacZ)286 was formerly called lacMS286
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- lacZ95=phi80dIIlacZ9 was formerly called lacBK1
- rbsD296::Tn10 was formerly called zie-296::Tn10
- xyl-7 was formerly called xylA7
- = tetracycline resistant
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: