CGSC Strain#: 5886
Strain Designation: F597/EB54     
Source of Strain: E.A. Birge
Sex: F'
Episome/Plasmid: F597
Plasmid Markers/Mutations: Chromosomal Markers: secA209(aziR)
,
pro-48,
lacZ118(Oc)
,
lacI22,
λ-,
trpA9605(Am)
,
his-85(Am)
,
gyrA19(NalR)
,
recA1,
rpsL171(strR)
,
metE90,
trpR55Strain Comments: - pro-48-- is probably in proB-.
- lacZ118(Oc)-- is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
- trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
- his-85(Am)-- is an amber mutation. It is suppressed by supD32.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- metE90-- is probably metE70.
- trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
- his-85(Am) was formerly called his-29
- lacI22 was formerly called laci3-
- lacI22 was formerly called lacI3
- secA209(aziR) was formerly called azi-9
- Am = amber(UAG) mutation
- aziR = azide resistance
- NalR = Nalidix.acidResist.
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: