How We Ship Strains


The CGSC generally distributes strains on sterile filter disks that have been impregnated with a small amount of glycerolized culture and wrapped in sterile foil.


Cultures distributed on filter disks

Cultures are viable in this state for approximately 12-15 days at room temperature, but we suggest that you culture the strains as soon as possible upon their arrival. If for some reason there is a delay in reviving the strains, the best way to preserve them is to store the wrapped filter disks at -80 deg. C until you can revive them.

To resuscitate the strain, aseptically place the disk onto a suitable agar plate (typically LB agar). Then rehydrate the disk with a drop of sterile LB broth and isolation streak out from the fluid surrounding the disk to obtain isolated colonies. The filter disk can be left on the plate during incubation. The resulting isolated colonies can then be screened to check their genetic markers and antibiotic resistance phenotypes.


Carefully unwrap filter disk

Using sterile forceps transfer disk to agar plate

Rehydrate filter disk with 1 drop of broth

Isolation streak from moisture around disk

Because the strains are shipped on filter disks rather than plates, tubes or vials, they are often packaged in an envelope rather than a box. This reduces shipping costs and also often prevents the strains from having long delivery delays clearing customs. Shipping strains on filter disks rather than stab cultures has also been shown to significantly reduce the incidence of spontaneous mutations during transit (1).

Depending on the number of strains being shipped and the shipping destination, strains may be mailed in any one of the three kinds of envelopes shown below:

It has always been our policy to replace, at no cost, any cultures that cannot be revived upon delivery.


References:

1) Spira, B, R de Almeida Toledo, RP Maharjan, T Ferenci 2011. The uncertain consequences of transferring bacterial strains between laboratories - rpoS instability as an example. BMC Microbiol. 11:248-.