Mutation - btuB754(del)::kan

Mutation - btuB754(del)::kanMutation btuB754(del)::kan

Mutation btuB754(del)::kan

Name: btuB754(del)::kan
Type: Insertion
Mutation of Gene: btuB
Approx. Map Location: 89.70
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were AGTCAAACACCAGCACGGTGGGACGTGGTTCAGAAGGTGTAGCTGCCAGAATTCCGGGGATCCGTCGACC and TATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Property	Symbol
deletion	del
kanamycin resistant