Mutation - fliC769(del)::kan

Mutation - fliC769(del)::kanMutation fliC769(del)::kan

Mutation fliC769(del)::kan

Name: fliC769(del)::kan
Type: Insertion
Mutation of Gene: fliC
Approx. Map Location: 43.11
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were GTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGATTCCGGGGATCCGTCGACC and GGAAACCCAATACGTAATCAACGACTTGCAATATAGGATAACGAATCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Property	Symbol
deletion	del
kanamycin resistant

3 Strains Carrying This Mutation

Name	Mutations	Genotype
WTZ2204	2	F-, λ^-, ΔfliC769::kan, rph-1
JW1908-1	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔfliC769::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW1908-1/pWTZ594	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔfliC769::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514, pWTZ594