Mutation frlR782(del)::kan
- Name: frlR782(del)::kan
- Type: Insertion
- Mutation of Gene: frlR
- Approx. Map Location: 75.48
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GATGCTGGCGCATCTTATCCGGCCTACGGTTAATGTCTTTTATTATCAATATTCCGGGGATCCGTCGACC and AACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5698-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔfrlR782::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514